Production of Membrane Proteins: Strategies for Expression by Anne Skaja Robinson PDF

By Anne Skaja Robinson

Designed as a research-level advisor to present concepts and strategies of membrane protein construction at the small to intermediate scale, this practice-oriented booklet presents distinct, step by step laboratory protocols in addition to an evidence of the rules at the back of each one approach, including a dialogue of its relative merits and downsides.
Following an introductory part on present demanding situations in membrane protein creation, the e-book is going directly to examine expression platforms, rising equipment and methods, and protein particular considerations.
Case reviews illustrate the best way to decide on or pattern the optimum construction procedure for any wanted membrane protein, saving either money and time at the laboratory in addition to the technical creation scale.
exact in its insurance of "difficult" proteins with huge membrane-embedded domain names, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

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Production of Membrane Proteins: Strategies for Expression and Isolation by Anne Skaja Robinson

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